RTRAF Knockout cell line (Hep G2)

RTRAF Knockout cell line (Hep G2) Order

Catalog#

YKO-HT7518

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

RTRAF Knockout cell line (Hep G2)

Species

Human

Gene Name

RTRAF

Gene ID

51637

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Target region

Knockout region: Exon 3 (based on transcript RTRAF-201)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the RTRAF gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, RTRAF gene knockout RTRAF Knockout cell line (Hep G2) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

RTRAF Knockout cell line (Hep G2)

Catalog#

YKO-HT7518

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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RTRAF Knockout cell line (Hep G2)

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